KMID : 0861020160310050071
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Korea Journal of Herbology 2016 Volume.31 No. 5 p.71 ~ p.78
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Wiryeongtang attenuates diabetic renal dysfunction in human renal mesangial cells
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Yoon Jung-Joo
Han Byung-Hyuk Choi Eun-Sik Namgung Seung Jeong Da-Hye Kim Hye-Yoom Ahn You-Mee Lee Yun-Jung Kang Dae-Gill Lee Ho-Sub
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Abstract
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Objectives : Diabetic nephropathy is one of the most common chronic complications of diabetes and a leading cause of end-stage renal failure in the world. Mesangial cell proliferation is known as the major pathologic features such as glomerulosclerosis and renal fibrosis. Wiryeongtang (WRT) is a well-known traditional herbal formula as therapeutic agents for chronic edema and dysuresia of renal homeostasis. In the present study, we investigated whether WRT inhibits high glucose (HG)-induced renal dysfunction by TGF-¥â/Smads signal regulation in cultured mesangial cells.
Methods : Inhibitory effect of WRT (10-50 §¶/ml) on HG-stimulated mesangial cells proliferation and dysfunction were evaluated by [3H]-thymidine incorporation, Western blot, and RT-qPCR.
Results : WRT significantly decreased HG-accelerated thymidine incorporation in human renal mesangial cell in a dose-dependent levels. WRT induced down-regulation of cyclins/CDKs and up-regulation of CDK inhibitor, p21waf1/cip1 and p27kip1 expression. In addition, HG enhanced expression of dysfunction biomarker such as collagen IV and CTGF, which was markedly attenuated by WRT. WRT decreased TGF-¥â1 and Smad-2/Smad-4 expression, whereas increased Smad-7 expression under HG. Furthermore, WRT inhibited HG-induced inflammatory factors level such as ICAM-1 and MCP-1 as well as NF-¥êB p65 nuclear translocation and intracellular ROS production.
Conclusions : These results suggested that WRT may alleviate mesangial proliferation and inflammation possibly involved in renal fibrotic process, further diabetic nephropathy through disturbing TGF-¥â1/Smad signaling and NF-¥êB/ROS pathway. Thus, WRT might prove to be effective in the treatment of renal dysfunction leading to diabetic nephropathy.
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KEYWORD
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Wiryeongtang, diabetic nephropathy, mesangial cell, TGF-¥â1, renal dysfunction
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